Effect on immune status after infectious bronchitis (IBv) vaccination when Aromax (XVET) is applied on broiler chicken

Type of trial: University Trial 
Location of trial: Iraq
Experimented animals: 210 one-day-old broiler chicken (Ross 308)
Trial aim: Assess the effect of XVET Germany’s AROMAX on immune status against Infectious bronchitis virus (IBv) vaccine on broiler chicken 
Original published paper: Amjed H Ulaiwi – Iraqi Journal of Agricultural Sciences –2019:50(6):1630-1634

Introduction to the study:
This study was performed at the University of Baghdad in Iraq to evaluate the effect of XVET Germany’s Aromax on the antibody titer formation when applied along with the vaccination of infectious bronchitis virus (IBv). Various administration routes from Aromax as well as the vaccination were tested to establish the best combination solution. 

Material and methods:

A total of 210 one-day-old broiler chicks (Ross -308) were separated randomly into 7 equal groups, all groups were kept in separated cages (3 m2) during the complete experiments period (35 days).

Depending on the trial design, the chicks were given:

• Aromax
  • In the drinking water (0.2 ml/L) every day or applied Aromax as a spray (2 ml/L) twice a week. In group 7 Aromax was not provided.
• Infectious Bronchitis virus (IBv) disease vaccination
  • In some groups chicks were vaccinated against IBv disease on day 15, 25, and 35 of age, using a live attenuated vaccine (1000 dose IBv-H120) given either orally or via spray. In groups 5 and 6 animals were not vaccinated. 

Blood samples were randomly collected from 10 chicks per group to measure the titer of IBv antibodies on days 15, 25, and 35. An antibody measurement was performed on days 15, 25, and 35 using ProFLOK® IBv ELISA Kit with ELISA Reader.

All statistical analysis was realized setting a p-value of P<0.05.

Results
The use of Aromax by a different route of administration activated the humoral immune response to the IBv vaccine as shown in Figure 1.

The result of IBv titer at 15 days old shows that the groups with a spray vaccination and Aromax spray (G4 and G2) present respectively a high significant difference than other groups. 

On day 25, G4 showed a highly significant difference among groups G1, G2, G3, than control groups (G5, G6, and G7) respectively.

On day 35, G4 had a highly significant difference among groups G1, G2, G3 than control groups (G5, G6, and G7) respectively.

Conclusion
The result of IBv titer showed that the usage of Aromax on drinking water and spray along the spray IBv vaccination (Group 4), and Aromax as spray along with IBV spray application (Group 2) had a significant better antibody production titer among the different groups. 

Concluding that the Aromax (either drinking water or spray application) helped to reach the best antibody titer against Infectious Bronchitis virus (IBv).

Acknowledgment
We would like to thank the author Amjed H. Ulaiwi for providing us with the study and the permission for the usage of the results as technical material.

For more information please contact technical@xvetgermany.com